案例信息

标题：见微知著，纳米医学的应用愿景与挑战——超顺磁氧化铁纳米颗粒(SPIO-NPs)的肝脏毒性测试和安全性评价

【专业学位类别】公共卫生

【专业领域/方向】卫生毒理学

【作者】林育纯

【单位】厦门大学公共卫生学院

【关键词】纳米医学、超顺磁氧化铁纳米颗粒、肝脏毒性、安全性评价

【适用课程】毒理学教程、现代实验预防医学

【版权说明】真实性案例，仅用于教学目的

【案例摘要】

  纳米医学基于纳米尺度的医用材料与器件的研发，广泛应用于疾病的预防、诊断、治疗和监测，实现纳米科技助力精准医学。因此，要实现纳米医学的临床转化和应用愿景，其安全性评价将成为纳米医学重点考量的公共卫生学问题。本案例基于课题组已建立的毒性测试和安全性评价平台，介绍已开展的纳米医用材料SPIO-NPs相关毒性检测设计与转化毒理学平台建设实践。该研究采用组学大数据挖掘、纳米材料表征、体内和体外试验，建立SPIO-NPs暴露介导肝细胞和肝组织毒理学效应测试和安全性评价平台。结果显示，较高剂量SPIO-NPs (20 mg/kg bw)急性暴露诱发肝细胞线粒体依赖性凋亡和肝脏毒性损伤。通过解读该研究毒性测试的关键环节，使学生熟悉纳米转化毒理学概念和基本内容，学习利用现代毒理学设计研究方案，培养学生开展毒性测试和安全性评价的能力。

Title: Hepatotoxicity testing and safety evaluation of superparamagnetic iron oxide nanoparticles (SPIO-NPs)

Professional Degree Category: Master of Public Health

Specialized fields: Health toxicology

Authors: Yu-Chun Lin

Affiliation: School of Public Health, Xiamen University

Abstract

Nanomedicine is based on the research and development of nanoscale medical materials and devices. These materials and devices are widely used in the prevention, diagnosis, treatment, and monitoring of diseases, as well as in the realization of nanotechnology-assisted precision medicine. Therefore, in order to achieve the goal of clinical translation and application of nanomedicine, the safety evaluation of nanomedicine will become a crucial public health concern. Based on the toxicity testing and safety evaluation platform already established by the group, this case study introduces the practice of designing the toxicity testing and translational toxicology platform for nanomedical materials, specifically SPIO-NPs. The study utilized histological big data mining, nanomaterial characterization, in vivo and in vitro tests to establish a platform for testing and evaluating the safety of SPIO-NPs exposure on hepatocytes and liver tissues, specifically focusing on their toxicological effects. The results showed that acute exposure to higher doses of SPIO-NPs (20 mg/kg bw) induced mitochondria-dependent apoptosis and hepatotoxic injury in hepatocytes. By examining the essential elements of toxicity testing in this study, students will become acquainted with the principles and fundamental components of nano-translational toxicology. They will also learn how to create research protocols using contemporary toxicology methods and acquire the skills to perform toxicity testing and safety assessments.

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