Dai Wang, PhD

Professor (Associate) / PhD Supervisor

1. Research interests：

I am interested in bacterial pathogenesis of Enterohemorrhagic Escherichia coli (EHEC）, Group B Streptococcus（GBS）and Mycobacterium tuberculosis (Mtb). Also I want contribute to the discovery of potential therapeutic targets for bacterial infection based on our previous findings.

Research interests include：

（1）Expression/translocation regulation of bacterial secretion system；

（2）Targets and mechanism of bacterial virulence inhibitor and antimicrobial agents；

（3）Host-pathogen interactions.

I was involved in different projects supported by BBSRC, MRC, Wellcome Trust, NIH and MRS，experienced in bio-safety lab practice（BSL-2，BSL-3）. I am now supported by NSFC and other chinese funding bodies and our findings were published on Proc Natl Acad Sci U S A、Cell Host & Microbe、J Biol Chem、Nucleic Acids Res、Trends Microbiol、Mol Microbiol、Antimicrob Agents Chemother. Especially，our published work (J Biol Chem 2011) regarding T3SS inhibitor (Salicylidene Acylhydrazide) was “Recommended” by Faculty of 1000.

2. Education:

PhD, College of Medicine and Veterinary Medicine, University of Edinburgh (The Roslin Institute);

MSc by Research, College of Medicine and Veterinary Medicine, University of Edinburgh;

BEng，School of Life Sciences, Shanghai University.

3. Work experiences:

Professor (Associate)，School of Public Health, Xiamen Univ.

Postdoctoral Research Fellow, College of Medicine and Veterinary Medicine/Roslin Institute, University of Edinburgh

Postdoctoral Research Associate, Department of Microbiology, University of Glasgow

Visiting Research Associate Scientist, Department of Molecular Biology and Microbiology, University of Massachusetts Medical School, MA, USA

Research Assistant, Department of Medical Microbiology, Medical School, College of Medicine and Veterinary Medicine, University of Edinburgh

4. Grants：

NSFC (PI): 81301474 / 31370166 / 31741006 / 81971905 / 82272349;

NSF of Fujian Province (PI): 2015J01345/2023J01040;

Xiamen University Chancellor fellowship (PI): 20720160060;

Early-stage Project of National Key Basic Research Program of China (Co-PI): SQ2015CB050735

5. Courses：

Undergraduate courses (Medical Microbiology; Molecular Biology; Sanitary Microbiology; Experimental Practice of Medical Microbiology);

Postgraduate courses (Introduction of translational medicine）

6. Membership:

Society for General Microbiology (SGM)

American Society for Microbiology (ASM)

7. Selected publications：

1. Synbiotic intervention with Bifidobacterium bifidum and prebiotics reduces enterohemorrhagic Escherichia coli colonization.Lett Appl Microbiol. 2026 Feb 4;79(2):ovag019. doi: 10.1093/lambio/ovag019.

2. Broad-spectrum tolerance to disinfectant-mediated bacterial killing due to mutation of the PheS aminoacyl tRNA synthetase. Proc Natl Acad Sci U S A, 2025 Feb 11;122(6):e2412871122.

3. Complete genome of Streptococcus agalactiae strain XM_1.Microbiol Resour Announc. 2024 Oct 10;13(10):e0032824. doi: 10.1128/mra.00328-24.

4. Dissecting and tracing the gut microbiota of infants with botulism: a cross sectional and longitudinal study.Front Microbiol. 2024 May 31:15:1416879. doi: 10.3389/fmicb.2024.1416879.

5. L-glutamine protects against enterohemorrhagic Escherichia coli infection by inhibiting bacterial virulence and enhancing host defense concurrently. Microbiol Spectr, 2023 Oct 10:e0097523. doi: 10.1128/spectrum.00975-23.

6. A broadly applicable, stress-mediated bacterial death pathway regulated by the phosphotransferase system (PTS) and the cAMP-Crp cascade. Proc Natl Acad Sci U S A, 2022 Jun 7; 119(23): e2118566119.

7. In Situ Live Imaging of Gut Microbiota. mSphere, 2021, 6(5): 0-e0054521

8. Gain-of-Function Mutations in Acid Stress Response (evgS) Protect Escherichia coli from Killing by Gallium Nitrate, an Antimicrobial Candidate. Antimicrob Agents Chemother. 2021 Feb 17;65(3):e01595-20. doi: 10.1128/AAC.01595-20. Print 2021 Feb 17.

9. Spirulina-Templated Porous Hollow Carbon@Magnetite Core-Shell Microswimmers. Applied Materials Today. 2021 March, Volume 22.

10. F0F1 ATP synthase regulates extracellular calcium influx in human neutrophils by interacting with Cav2.3 and modulates neutrophil accumulation in the lipopolysaccharide-challenged lung. Cell Commun Signal. 2020 Feb 4;18(1):19. doi: 10.1186/s12964-020-0515-3.

11. Beyond a Ribosomal RNA Methyltransferase, the Wider Role of MraW in DNA Methylation, Motility and Colonization in Escherichia coli O157:H7. Front Microbiol. 2019 Nov 13;10:2520. doi: 10.3389/fmicb.2019.02520. eCollection 2019.

12. An RNA-dependent mechanism for transient expression of bacterial translocation filaments. Nucleic Acids Res. 2018 Feb 8. doi: 10.1093/nar/gky096. [Epub ahead of print]

13. Dimethyl Sulfoxide Protects Escherichia coli from Rapid Antimicrobial-Mediated Killing. Antimicrob Agents Chemother. 2016 Aug;60(8):5054-8.

14. Identification of Bacterial Target Proteins for the Salicylidene Acylhydrazide Class of Virulence- blocking Compounds. J Biol Chem. 2011 Aug 26;286(34):29922-31. Epub 2011 Jul 1. PubMed PMID: 21724850. (Faculty of 1000 “Recommended”)

15. Hierarchal type III secretion of translocators and effectors from Escherichia coli O157:H7 requires the carboxy terminus of SepL that binds to Tir. Mol Microbiol. 2008 Sep;69(6):1499-512. Epub 2008 Jul 30. PubMed PMID: 18673458.

16. Expression, purification, crystallization and initial X-ray diffraction analysis of thiol peroxidase from Yersinia pseudotuberculosis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1606-9. Epub 2010 Nov 25. PubMed PMID: 21139206; PubMed Central PMCID: PMC2998365.

17. Characterization of the effects of salicylidene acylhydrazide compounds on type III secretion in Escherichia coli O157:H7. Infect Immun. 2009 Oct;77(10):4209-20. Epub 2009 Jul 27. PubMed PMID: 19635828; PubMed Central PMCID: PMC2747932.

18. Salmonella Transforms Follicle-associated Epithelial Cells into M cells to Promote Intestinal Invasion. Cell Host & Microbe, 2012 Nov 15 ; Vol 12, pp. 645–656.

19. A Highly Conserved Bacterial D-Serine Uptake System Links Host Metabolism and Virulence. PLoS Pathog. 2016 Jan 4;12(1):e1005359. doi: 10.1371/ journal.ppat.1005359. eCollection 2016 Jan.

20. Type III Secretion-Dependent Sensitivity of Escherichia coli O157 to Specific Ketolides. Antimicrob Agents Chemother. 2015 Nov 2; 60, 459-470, doi:10.1128/aac.02085-15.

21. The metabolic enzyme AdhE controls the virulence of Escherichia coli O157:H7. Mol Microbiol. 2014 Jul;93(1):199-211. doi: 10.1111/mmi.12651. Epub 2014 Jun 9. PubMed PMID: 24846743; PubMed Central PMCID: PMC4249723.

22. Controlling injection: regulation of type III secretion in enterohaemorrhagic Escherichia coli. Trends Microbiol. 2009 Aug;17(8):361-70. Epub 2009 Aug 5. Review. PubMed PMID: 19660954.

23. Expression and Regulation of the Escherichia coli O157:H7 Effector Proteins NleH1 and NleH2. PLoS ONE. 2012 Mar 12;7(3): e33408. doi: 10.1371/journal.pone.0033408.

24. Mucosal antibody responses of colonized cattle to Escherichia coli O157-secreted proteins, flagellin, outer membrane proteins and lipopolysaccharide. FEMS Immunol Med Microbiol. 2008 Jan;52(1):59-68. Epub 2007 Nov 8. PubMed PMID: 17995963.

25. Analysis of the expression, regulation and export of NleA-E in Escherichia coli O157 : H7. Microbiology. 2007 May;153(Pt 5):1350-60. PubMed PMID: 17464049.

26. Structural characterisation of Tpx from Yersinia pseudotuberculosis reveals insights into the binding of salicylidene acylhydrazide compounds. PLoS ONE. 2012 Feb 27;7(2): e32217.doi:10.1371/journal.pone. 0032217 .

27. PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP Pathway. Anal Cell Pathol (Amst). 2019 Jan 23;2019:7145198. doi: 10.1155/2019/7145198. eCollection 2019.

28. Correlation between the protein expression levels of A-kinase anchor protein95, p-retinoblastoma (Ser780), cyclin D2/3, and cyclin E2 in esophageal cancer tissues. Asia Pac J Clin Oncol. 2019 Oct;15(5):e162-e166. doi: 10.1111/ajco.13146. Epub 2019 Apr 16.

29. Epac1 is involved in cell cycle progression in lung cancer through PKC and Cx43 regulation. Folia Histochem Cytobiol. 2018;56(1):21-26. doi: 10.5603/FHC.a2018.0004. Epub 2018 Mar 12.

30. Epac1, PDE4, and PKC protein expression and their correlation with AKAP95 and Cx43 in esophagus cancer tissues. Thorac Cancer. 2017 Nov;8(6):572-576. doi: 10.1111/1759-7714.12479. Epub 2017 Aug 3.

31. Epac1, PDE4, and PKC protein expression and their association with AKAP95, Cx43, and cyclinD2/E1 in breast cancer tissues. Thorac Cancer. 2017 Sep;8(5):495-500. doi: 10.1111/1759-7714.12475. Epub 2017 Jul 29.